Figure 7.

ID and radiations-induced VEGF mRNA up-regulation depends on cellular ROS production from different origins. Cells were cultured in iodine-containing medium for 7 days. Medium was replaced by iodine-containing (control) or iodine deficient (ID) medium and cells were then irradiated with a dose of 3 Gy. Part of the cells was treated with DPI (A,B) or mitoTEMPO (C,D). Cells were harvested 4 or 6 h after medium change. VEGF mRNA expression in MCF12A (A, C) and MCF7 cells (B, D) was determined using RT-qPCR. Data are expressed as means ± SEM. p-value < 0.05 were considered as statistically significant. *p < 0.05, **p < 0.01, ***p < 0.001. Statistical test: one-way ANOVA with Newman–Keuls post-hoc test. A: N = 5; B, D: N = 3; C: N = 6.