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. 2020 May 31;21(11):3963. doi: 10.3390/ijms21113963

Figure 8.

Figure 8

ID and radiation additively up-regulate antioxidant enzymes mRNA in breast cells. Cells were cultured in iodine-containing medium for 7 days. Medium was replaced by iodine-containing (control) or iodine deficient (ID) medium and cells were then irradiated with a dose of 0.1 or 3 Gy. Cells were harvested 3 (MCF12A) or 6 (MCF7) hours after medium change. SOD1, SOD2, and catalase mRNA expression in MCF12A (A–F) and MCF7 cells (G–L) was determined using RT-qPCR. Data are expressed as means ± SEM. p-value < 0.05 were considered as statistically significant. *p < 0.05, **p < 0.01. Statistical test: one-way ANOVA with Tukey post-hoc test. A: N = 6; B, G: N = 4; C, D, F, K: N = 5; E: N = 8; H, I, J, L: N = 3.