Skip to main content
. 2020 May 31;21(11):3963. doi: 10.3390/ijms21113963

Table 1.

Primer sequences and their annealing temperature for qRT-PCR.

RNA Forward Primer Reverse Primer Annealing Temp.
β-actin 5′CATCCTGCGTCTGGACCT3′ 5′AGGAGGAGCAATGATCTTGAT3′ 62 °C
VEGF-A 5′GCAGATGTCCCGGCGAAGAGAAGA3′ 5′CGGGGAGGGCAGAGCTGAGTGTTA3′ 62 °C
VEGF165 5′GAGCAAGACAAGAAAATCCC3′ 5′CCTCGGCTTGTCACATCTG3′ 58.5 °C
VEGF165b 5′GAGCAAGACAAGAAAATCCC3′ 5′GTGAGAGATCTGCAAGTACG3′ 59 °C
SOD1 5′GCGTGGCCTAGCGAGTTAT3′ 5′TTTGCCCAAGTCATCTGCT3′ 56 °C
SOD2 5′GTTGGGGTTGGCTTGGTTTC3′ 5′ACGATCGTGGTTTACTTTTTG3′ 52 °C
Catalase 5′GCAAACCGCACGCTATGG3′ 5′ACGAGGGTCCCGAACTGTGT3′ 55 °C