Table 1.
RNA | Forward Primer | Reverse Primer | Annealing Temp. |
---|---|---|---|
β-actin | 5′CATCCTGCGTCTGGACCT3′ | 5′AGGAGGAGCAATGATCTTGAT3′ | 62 °C |
VEGF-A | 5′GCAGATGTCCCGGCGAAGAGAAGA3′ | 5′CGGGGAGGGCAGAGCTGAGTGTTA3′ | 62 °C |
VEGF165 | 5′GAGCAAGACAAGAAAATCCC3′ | 5′CCTCGGCTTGTCACATCTG3′ | 58.5 °C |
VEGF165b | 5′GAGCAAGACAAGAAAATCCC3′ | 5′GTGAGAGATCTGCAAGTACG3′ | 59 °C |
SOD1 | 5′GCGTGGCCTAGCGAGTTAT3′ | 5′TTTGCCCAAGTCATCTGCT3′ | 56 °C |
SOD2 | 5′GTTGGGGTTGGCTTGGTTTC3′ | 5′ACGATCGTGGTTTACTTTTTG3′ | 52 °C |
Catalase | 5′GCAAACCGCACGCTATGG3′ | 5′ACGAGGGTCCCGAACTGTGT3′ | 55 °C |