Figure 1.

Ang-(1-7) prevents the decrease of muscle function in mice fed with the DDC diet. Male mice C57BL/6J (16 weeks old) were randomly separated into four groups defined as Control, Ang-(1-7), DDC, and DDC + Ang-(1-7). Ang-(1-7) was administered through osmotic pumps. The mice were fed for six weeks with a standard diet or with a DDC-supplemented diet. (A) At week 0 and week 6, mice were assessed for muscle strength measured by weightlifting test. Values represent a score which is normalized per body weight. (B) Running test results was expressed as the number of detentions in the treadmill. (C) Performance in the running test was determined as the time spent in the Low-Performance zone (LP) and High-Performance zone (HP). Values in A, B, and C are shown as mean ± SEM. Five or six animals per group were used for three independent experiments (* p < 0.05 vs. Control, # p < 0.05 vs. DDC. Two-way ANOVA, Tukey’s multiple comparisons test).