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. 2020 May 29;21(11):3891. doi: 10.3390/ijms21113891

Figure 4.

Figure 4

Ang-(1-7) prevents the fiber type transitions in gastrocnemius muscle from mice fed with the DDC diet. Male mice C57BL/6J (16 weeks old) were randomly separated into four groups: Control, Ang-(1-7), DDC, and DDC + Ang-(1-7). DDC was administrated by diet for six weeks, whereas Ang-(1-7) was administered through osmotic minipumps for six weeks. In each experiment, five mice were used for each experimental condition. (A) At the end of the experiment, mice were sacrificed, and cryosections of gastrocnemius muscles were obtained. The fiber type was determined through immunofluorescent detection of myosin heavy chain isoforms (IIA, IIB, I). Scale bar: 120 µm. (B) Quantitative analysis of the fiber type was plotted and shown as the percentage of fiber types relative to the total fibers per field. Values represent the mean ± SEM. (* p < 0.05 vs. Control, # p < vs. DDC. (* p < 0.05 vs. Control; # p < vs. DDC. One-way ANOVA, Tukey’s multiple comparisons test). (C) The color map represents the abundance of different fiber types in each condition, from low (blue) to high (yellow) grade.