Figure 5.

Preventive effect of Ang-(1-7) on decreased myosin heavy chain (MHC) protein levels in gastrocnemius from DDC-treated mice. C57BL/6J male mice were fed for six weeks with a standard diet (Control) or DDC-supplemented diet (DDC) with or without Ang-(1-7) (through osmotic pumps). For three independent experiments, five mice were used for each experimental condition. (A) At the end of 6 weeks the mice were sacrificed, gastrocnemius muscle was removed, and protein extraction was performed to detect MHC protein levels by western blot. β-actin was used as the loading control, and molecular weights are depicted in kilodaltons. (B) Densitometric analysis of the bands is expressed as fold of induction relative to control. Values represent the mean ± SEM. (* p < 0.05 vs. Control, # p < vs. DDC. One-way ANOVA, Tukey’s multiple comparisons test).