Figure 10.

Protein profile of cell cycle regulating proteins (Rictor, Raptor, histone acetylation, p19, p27) after the long-term application of 0.5 nM everolimus (E) or 2.5 µM sulforaphane (S) or 0.5 nM everolimus + 2.5 µM sulforaphane (E + S) to synchronized RT112 tumor cells. Controls (C) received cell culture medium alone. One representative of three separate experiments is shown. Each protein analysis was accompanied by a β-actin loading control. One representative internal control is shown. The ratio of protein intensity/β-actin intensity is expressed as percentage of controls, set to 100%. * indicates significant difference to controls, p ≤ 0.05.