Figure 2.

1,2-NQ and 1,4-NQ induce CYP1A1/cyp1a1 mRNA in mouse and human hepatoma cells. Human HG2L7.5c.1 and mouse H1L7.5c.3 hepatoma cells were incubated with DMSO (1% v/v), 1,2-NQ (5 nM–5 μM) or 1,4-NQ (5 nM–5 μM). After 4h incubation, cells were harvested with TRIzol reagent, RNA isolated, cDNA reverse transcribed and CYP1A1/1a1 mRNA transcripts quantitated by qPCR as described under Materials and methods. CYP1A1 and cyp1a1 transcript was normalized to beta-actin reference gene control and background DMSO was subtracted. Values were expressed as the mean ± standard deviation of triplicates of three biological replicates cells and an asterisk (*) indicates those values that were significantly different from DMSO treated cells at p < 0.05 as determined by Two-Way ANOVA.