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. 2020 May 28;21(11):3827. doi: 10.3390/ijms21113827

Figure 6.

Figure 6

Adhesion forces of SCP1-HAS-eGFP and SCP1-mock to Col-I and BSA coated surfaces. Cells were incubated 24 h in culture medium containing 10 mM GlcNAc prior to AFM measurements. The experiments were performed in serum-free HEPES-buffered MEM α medium with 10 mM GlcNAc at 37 °C. (A–D) Representative force-distance curves for each of the cell lines; vertical lines indicate single step positions. (E) Average step heights show the mean force necessary to detach one adhesive event. (F) Steps per curve indicate the mean number of adhesive events per cell. (G) The overall adhesive force per cell is shown by the total step height per curve. (H) The detachment force is defined as the global maximum of a detachment force curve. (I) Work of detachment was calculated as the integral between the detachment force-distance curve and its corresponding base line. Error bars represent SD, the asterisks indicate a p-Value < 0.05 (*) or < 0.01 (**).