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. 2020 May 28;21(11):3827. doi: 10.3390/ijms21113827

Table 1.

PCR Primers.

Primer Sequence Annealing Temperature
HAS1_for 5′-GACTCCTGGGTCAGCTTCCTAAG-3′ 55 °C
HAS1_rev 5′-AAACTGCTGCAAGAGGTTATTCCT-3′ 55 °C
HAS2_for 5′-CATAAAGAAAGCTCGCAACACG-3′ 55 °C
HAS2_rev 5′-ACTGCTGAGGAATGAGATCCAG-3′ 55 °C
HAS3_for 5′-GACGACAGCCCTGCGTGT-3′ 58 °C
HAS3_rev 5′-TTGAGGTCAGGGAAGGAGAT-3′ 58 °C
GFP_for 5′-CAAGCTGACCCTGAAGTTCATCTGC-3′ 50 °C
GFP_rev 5′-CACGCTGCCGTCCTCGATGTTGTGG-3′ 50 °C
GAPDH_for 5′-CAACTACATGGTTTACATGTTC-3′ 50 °C
GAPDH_rev 5′-GCCAGTGGACTCCACGAC-3′ 50 °C