Table 1.
Primer | Sequence | Annealing Temperature |
---|---|---|
HAS1_for | 5′-GACTCCTGGGTCAGCTTCCTAAG-3′ | 55 °C |
HAS1_rev | 5′-AAACTGCTGCAAGAGGTTATTCCT-3′ | 55 °C |
HAS2_for | 5′-CATAAAGAAAGCTCGCAACACG-3′ | 55 °C |
HAS2_rev | 5′-ACTGCTGAGGAATGAGATCCAG-3′ | 55 °C |
HAS3_for | 5′-GACGACAGCCCTGCGTGT-3′ | 58 °C |
HAS3_rev | 5′-TTGAGGTCAGGGAAGGAGAT-3′ | 58 °C |
GFP_for | 5′-CAAGCTGACCCTGAAGTTCATCTGC-3′ | 50 °C |
GFP_rev | 5′-CACGCTGCCGTCCTCGATGTTGTGG-3′ | 50 °C |
GAPDH_for | 5′-CAACTACATGGTTTACATGTTC-3′ | 50 °C |
GAPDH_rev | 5′-GCCAGTGGACTCCACGAC-3′ | 50 °C |