Table 2.
The primer sequences and reaction conditions for real-time PCR.
| Gene | Oligonucleotide Sequence 5′→ 3′ | Annealing Temp. (°C) | PCR Product Size (bp) | RefSeq | Ref. |
|---|---|---|---|---|---|
| HTRA1 |
Forward: CGGAAGATGGACTGATCGTGAC Reverse: GGTGATGGCTTTTCCTTTGGCC |
62 | 506 | NM_002775.4 | [42] |
| HTRA2 |
Forward: CCCTATCTCGAACGGCTCAGG Reverse: CCATGCTGAACATCGGGAAAGC |
63 | 639 | NM_013247.4 | [42] |
| HTRA3L |
Forward: AGATCAAAGACTGGAAGAAGCG Reverse: ATGATGTCACCATCTTGGATGC |
60 | 181 | NM_053044.1 | This work |
| HTRA3S |
Forward: ATCCATCCCAAGAAAAAGCTCC Reverse: CTCAATGAACTGCCAGTGAGG |
60 | 130 | NM_001297559.1 | This work |
| PPIA |
Forward: CTTGGGCCGCGTCTCCTTTGAG Reverse: GCTTGCCATCCAACCACTCAGTC |
59 | 329 | NM_001300981.1 | [68] |
| IPO8 |
Forward: TTGGAAGAAACCGCGCTTGAGG Reverse: ACCAGGCTGCATCTCGACTCTG |
59 | 118 | NM_001190995 | [68] |