Table 4.
qPCR Primers.
| miRNA | Primer Sequence 5′ to 3′ a | R2 | Amplification Efficiency (%) |
|---|---|---|---|
| miR-155-5p b | TTAATGCTAATCGTGATAGGGGT | 0.999 | 81.3 |
| miR-146b-5p | TGAGAACTGAAGTCCATAGATGG | 0.986 | 104.6 |
| miR-146a-5p b | TGAGAACTGAATTCCATAGATGG | 0.989 | 115.9 |
| miR-126-3p | TCGTACCGTGAGTAATAATGCA | 0.984 | 107.2 |
| miR-150-5p | TCTCCCAATCCTTGTACCAGTG | 0.992 | 113.9 |
| miR-2188-3p | GCTGTGTGAGGTCAGACCTATC | 0.982 | 116.5 |
| miR-139-5p | TCTACAGTGCATGTGTCTCCAGT | 0.974 | 100.9 |
| miR-221-5p | ACCTAGCATACAATGTAGATTTC | 0.984 | 115.4 |
| miR-200ae-3p | TAATACTGCCTGGTAATGATGAT | 0.952 | 82.3 |
| Normalizers | |||
| miR-125a-5p | TCCCTGAGACCCTAACTTGTGA | 0.994 | 115.1 |
| miR-19c-3p | TGTGCAAATCCATGCAAAACTG | 0.990 | 104.1 |
a Mature miRNA sequences were used as the forward specific primer, whereas a universal primer was used as a reverse primer. All primers showed no amplification in the no-template controls and generated an amplicon with a single melting peak. b 4-point serial dilution curve was used.