Skip to main content
. 2020 Jun 4;21(11):4034. doi: 10.3390/ijms21114034

Figure 3.

Figure 3

Native Sjp40 and Sjp90α identified in eggs and egg secreted proteins (ESP) of S. japonicum. Immunolocalization of Sjp40 and Sjp90α in eggs trapped in infected mouse liver probed with mouse anti-rSjp40 and anti-rSjp90 antibodies, respectively. Sections of livers from mice infected with S. japonicum were incubated with naïve control mouse serum (negative control) and mouse anti-rSjp40 or anti-rSjp90α antiserum, and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibody (a) or Alexa-Fluor 647-conjugated secondary antibody (b). DAPI stained nuclei are blue. A section of schistosome eggs in a developing granuloma shows SjP40 or Sjp90 staining (brown in HRP labelling and red in Alexa-Fluor 647) in eggs. NM—neural mass; EPI—epidermal cells. Scale-bars: 20 μm. (c,d) Western blotting was used to detect recombinant Sjp40 (c, left lane) and Sjp90α (d, left lane). Anti-rSjp40 (c, right lane) and anti-rSjp90 (d, right lane) were used to probe protein extracts of the soluble egg antigen (SEA) and egg secreted protein (ESP).