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NHEJ and SSTR analysis of modified ssODNs and protein addition. (A,C) The efficiency of NHEJ, SSTR, and SSTR/NHEJ ratio was analyzed using the different-sized ssODNs or NLS-tagged ssODNs. (B,D) NHEJ frequency calculated on Cdk1 or free NLS peptide treatment. Red region: 20 bp KI sequence. Black region: homologous sequence. HU: hydroxyurea, ND: nocodazole. Statistical analysis was performed using Student’s t-test. *: p < 0.05, **: p < 0.01, and ***: p < 0.001.
