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. 2020 Jun 11;21(11):4186. doi: 10.3390/ijms21114186

Figure 4.

Figure 4

CaCML13-silencing-impaired pepper immunity against RSI coupled with downregulation of PR genes. (a) Test of the success of CaCML13 silencing in TRV:CaCML13 pepper plant leaves challenged with RSI at the transcriptional level at 24 h post-inoculation; (b) bacterial wilt symptoms in the CaCML13 silenced and the control pepper plants at 11 days post inoculation by root-irrigation; (c) disease index from 5 to 14 dpi in CaCML13 silenced pepper and the control plants challenged with RSI; (d) growth of R. solanacearum displayed with cfu in CaCML13 silenced and the control pepper plant leaves challenged with RSI at 24 and 48 h post-inoculation; (e) relative transcript levels of immunity associated genes including CaPR1, CaNPR1 and CaDEF1 were significantly downregulated by CaCML13 silencing at 24 h post-inoculation. In (a,c–e), data presented are means ± standard error of four replicates. Different uppercase letters above the bars indicate significant differences among means (p < 0.01) by Fisher’s protected LSD test.