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. 2020 Jun 2;21(11):3991. doi: 10.3390/ijms21113991

Figure 7.

Figure 7

LRL2 inhibits the promoter activity of mild osmotic stress-responsive genes. (A) Diagrams of the dual-luciferase reporter and effector constructs. The EXT6, XTH14, AT3G49960, and TT6 promoters were used to drive firefly luciferase (Luc). 35Sp::Renilla luciferase (REN) was used as an internal control. For the effectors, the coding sequences of LRL2, AtSK11, and AtSK12 were fused to YFP or GFP driven by the 35S promoter. (B) Relative Luc/REN ratios in Nicotiana benthamiana mesophyll cells co-transfected with different Luc reporters and LRL2-YFP or YFP. (C) Relative Luc/REN ratios in N. benthamiana mesophyll cells co-transfected with the EXT6p::Luc reporter and LRL2-YFP, AtSK11-GFP, or both constructs. (D) Relative Luc/REN ratios in N. benthamiana mesophyll cells co-transfected with the EXT6p::Luc reporter and LRL2-YFP, AtSK12-GFP, or both constructs. Data are means ± SD. Student’s t-test was used to determine the significance of difference. Significant levels: *** p < 0.001.