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. 2020 Jun 10;21(11):4137. doi: 10.3390/ijms21114137

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Immunostaining for TUNEL (green) shows apoptotic cells in the molecular layer of the cerebellar cortex. (B) Nuclei were stained with PI in red. (C) Merged image of TUNEL and PI immunostaining. White arrowheads indicate TUNEL/PI-positive cells. (D) There was a significant increase in the number of TUNEL/PI-positive cells in the contralesional (left) cerebellar cortex compared to the ipsilesional (right) cerebellar cortex (scale bar: 50 μm, * p < 0.001, n = 11 in each group). TUNEL: terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling, PI: propidium iodide.