Figure 4.

Effect of HD and HT on rotenone induced locomotion impairment. 10 µM rotenone administration with and without simultaneous HD or HT treatment started at the fourth larval stage. The wave initiation rate (A), the body wave number (B) and the activity index (C) are shown at the third (A3) and seventh (A7) day of adulthood. Data are pooled from two biological repeats with ≥44 worms per treatment and age. The bars represent the mean ± SEM and differences compared to control were considered significant at p < 0.05 (*) and p < 0.001 (**). The appearance of single frames in swim analysis are shown before (D) and after (E) image processing. In addition, single nematodes recognized by CeleST (as indicated by the green outline) are shown with increasing number of body waves (F). The blue scale bars represent 2.5 mm (D,E) and 200 µm (F), respectively.