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. 2020 May 29;21(11):3896. doi: 10.3390/ijms21113896

Table 4.

List of primer sequences used for qRT-PCR of cytokinin-related genes. BrCRR1 is involved in protein phosphorylation and BrPYL1 is a receptor of ABA.

Arabidopsis Homolog (Accession Number) Gene Name BRAD ID cDNA Size (bp) Primer Forward (F) and Reverse(R) Product Size (bp)
At4g31920 BrRR1 Bra023972 1557 F:AGCTCAAGAACATATGGCAA 191
R:TGGATCATCGTTCTCATTCC
At2g01830 BrHK1 Bra024849 3123 F:AGCTCTGAAGAAGTTTGGAG 200
R:GTAAATGCCATTCCAGCTTC
At3g48100 BrRR2 Bra018084 540 F:TACTCAGAGTCTCTTCGTGT 171
R:CTTCTTGAGTAGTTCATATC
At1g10470 BrRR3 Bra036963 786 F:GTTCAGAGATGATGAGGGTC 208
R:GCAGATGCTTTCTCGTTGTC
At1g74890 BrRR4 Bra015885 558 F:TCTACCTCGGAGTTACATGT 161
R:CCAGAAGATCCTTTGTCTCC
At3g55950 BrCRR1 Bra007202 1023 F:GATTACTATGGGTGAGCTGG 167
R:CTCTCTCCAAATTTCCGACA
At1g28230 BrPUP1 Bra010890 1059 F:TAGCTTTCAACGCTCTCTTT 159
R:CAACCACATACTCCTTGTGA
At4g16420 BrADA1 Bra040578 1461 F:ATGAAATGCTTCTCCTGGAG 178
R:TTCTTGTTCTTCCCTGCTAC
At3g19160 BrIPT1 Bra001737 984 F:TTCTGAGCTGAGGTACGATT 194
R:ACTCCGGTACTCCTATAGCC
At3g57040 BrRR5 Bra003265 723 F:GGCATGACTGGTTATGATTT 237
R:AAGAGATTGAAGCACCTTCA
At1g19050 BrRR6 Bra016526 624 F:TTAGTTCGCCTGACCTACAT 163
R:TCAGAATCTCCTGTGTTTCC
At5g05440 BrPYL1 Bra009113 615 F:GAGAGGCTCGAGATCCTGGAC 237
R:ACAGCTTCTAGCCAGAGACTG
At2g19500 BrCKX2 Bra036719 1509 F:TTAGTGAAGAGCCACGGTAT 210
R:CCATAAACCCAAAGATCTGA
At5g56970 BrCKX3 Bra035640 1557 F:GGGAGAGCTAAACCTGAAAT 238
R:TAAGAACCCTACCGCATAAA
At4g29740 BrCKX4 Bra024135 1575 F:GCTGGTGGATCACATAACTT 170
R:ATTTTCCTCCTCGAGAAATC
At1g75450 BrCKX5 Bra015842 1575 F:GTATCTTTCCGTTGGTGGTA 200
R:CTCGTGTAATGATCCCAAAT
At3g63440 BrCKX6 Bra007743 1350 F:GGTTATCCTTCACACCAGAA 225
R:AGACATGTACCCTGTCCAAG
XM_009147610.2 ACTIN1 1371 F: CAACCAATCGTCTGTGACAA 105
R: ATGTCTTGGCCTACCAACAA
FJ969844.1 ACTIN2 491 F: AATGGTACCGGAATGGTCAA 119
R: TCCTTCTGGTTCATCCCAAC