Table 1.

SlitCSP3 residues from site 1 and site 2 showing interactions with secondary metabolites.

Site 1	Residues Forming Hydrogen Bonds	Residues Involved in Hydrophobic Interactions
DIMBOA	Arg32, Arg38, Lys84	Asp29, Lys41, Ala42, Lys45, Ile81
MBOA	Arg38, Lys84	Asp29, Arg32, Lys41, Ile44, Lys45, Tyr77, Ile81
Nicotine	Asp29	Arg38, Lys41, Ile44, Lys45, Tyr77, Gln80, Ile81, Lys84
Site 2
DIMBOA	Asp89	Trp78, Lys79, Leu82, Tyr85, Gln90, Tyr91
Nicotine	No H-bond formation	Trp78, Lys79, Ala83, Tyr85, Asp89, Tyr91