Figure 3.

Construction of overexpression and shRNA vector and transfection cells. (a) A549/DDP cells were transfected with three groups of RP11-838N2.3 shRNA vectors. The shRNA-2 and shRNA-3 expression levels were plainly lower than the NC and shRNA-1 groups, and the differences were statistically significant (P < 0.001). Among them, the shRNA-2 group has the highest interference efficiency. (b) The expression level of RP11-838N2.3 in LAD cisplatin-sensitive A549 cells transfected with the overexpression vector was plainly higher than that in the NC group (P < 0.01). ^∗∗P < 0.01; ^∗∗∗P < 0.001.