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. 2020 Jun 2;21(11):3993. doi: 10.3390/ijms21113993

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of the PdOLP1 protein. (a) Amino acid sequence of the coding region of PdOPL. The sequence highlighted in yellow indicates the THN domain. The AWS domain is boxed. The VWC domain is underlined with a thin black line. FU domain is underlined with double black line. SMART (http://smart.embl-heidelberg.de/) was used to analyze the PdOPL1 protein sequence. (b) A phylogenetic tree of the OPL proteins from 52 plants was constructed based on the neighbor-joining method, including PdOPL1 (GenBank:MK052942), PdOLP2: (Podel.01G248000.1), PagOLP1: (Pop_G09G014129.T1), PagOLP2:.(Pop_A01G056866.T1), PagOLP3: (Pop_A09G059600.T1), PagOLP4: (Pop_G01G020795.T1), PtrOLP (GenBank:XP_024463852.1), PeuOLP (GenBank:XP_011042680.1), PtoOLP (GenBank:APA20308.1), MeOLP (GenBank:XP_021626825.1), HbOLP (GenBank:XP_021636655.1), RcOLP (GenBank:XP_002531364.1), DzOLP (GenBank:XP_022756598.1), CjOLP (GenBank:BAI63297.1), VfOLP (GenBank:ARV78462.1), TcOLP (GenBank:XP_007016468.1), CsiOLP (GenBank:XP_006488293.1), CcOLP (GenBank:XP_006424795.1), VvOLP (GenBank:XP_002281193.1), HuOLP (GenBank:XP_021278215.1), JrOLP (GenBank:XP_018823302.1), QsOLP (GenBank:XP_023907784.1), GhOLP (GenBank:XP_016689054.1), GrOLP (GenBank:XP_012446484.1), GaOLP (GenBank:XP_017650093.1), JcOLP (GenBank:XP_012065012.1), EsOLP (GenBank:XP_006409911.1), RsOLP (GenBank:XP_018463081.1), BnOLP (GenBank:XP_013676421.1), BrOLP (GenBank:XP_009140942.1), BooOLP (GenBank:XP_013632142.1), BoOLP (GenBank:AAO12209.1), RcOLP (GenBank:XP_024159050.1), OeOLP (GenBank:XP_022881590.1), FaOLP (GenBank:ABB86299.1), CsOLP (GenBank:XP_010470049.1), ErgOLP (GenBank:XP_012827362.1), FvOLP (GenBank:XP_004294572.1), InOLP (GenBank:XP_019199014.1), NtOLP (GenBank:XM_018769102.1), NnOLP (GenBank:XP_010259948.1), ElgOLP (GenBank:XP_010918389.1), EgOLP (GenBank:XP_010033142.1), NtOLP (GenBank: NP_001312698.1), PaOLP (GenBank:XP_021820385.1), AtOLP (GenBank:NP_001324474.1), AlOLP (GenBank:XP_020885214.1), DcarOLP (GenBank:XP_017251860.1), NsOLP (GenBank:XP_009800616.1), CpOLP (GenBank:XP_021887635.1), GmOLP (GenBank:XP_003538000.1), CrOLP (GenBank:XP_006294865.1), NaOLP (GenBank:XP_019237711.1), MdOLP (GenBank:XP_008357503.1). DcatOLP (GenBank:XP_020683326.1), SlOLP (GenBank:NP_001234714.1). The 51 OPL amino acid sequences are list in Table S5. Bar = 0.05 substitutions per site.