Anatomical features of the stem cross-sections, growth, and wood property indexes of PdOLP1 transgenic plants. (a,b) PdOLP1-OE plants showed smaller shoots and a decreased stem diameter compared to WT plants. Plant height (a) and stem width (b) were measured at every fifth node from the apex. (c) Xylem widths (left panel) and numbers of xylem cell layers (right panel) in transgenic or WT plants. (d) Phloem widths (left panel) and numbers of phloem cell layers (right panel) in transgenic or WT plants. (e) Cambium widths (left panel) and numbers of cambium cell layers (right panel) in transgenic or WT plants. (f) The average internode lengths (left panel) and the xylem: phloem width ratios (right panel) in transgenic or WT lines. (g) Thicknesses of vessels and fibers in the stems of transgenic or WT plants. The values are the mean ± standard error (SE) of 20 cells. (h) Lignin content of transgenic or WT plants. (i) Analysis of the microfibril angle (MFA) in PdOLP1 transgenic poplar plants. The PdOLP1-DR poplar lines had lower MFAs than the control line. Significant differences between the transgenic plants and WT plants are marked with asterisks (* p < 0.05). WT: wildtype poplar; WT1: control for PdOLP1-OE plant; WT2: control for the PdOLP1-DR plant; OE3, OE12 and OE21: PdOLP1-OE poplar lines; DR8, DR15 and DR32: PdOLP1-DR plants.