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. 2020 Jun 2;21(11):3993. doi: 10.3390/ijms21113993

Figure 5.

Figure 5

Anatomical transverse sections of PdOLP1 transgenic poplar. The 15th internodes of 1.5-year-old plants were used to observe the anatomical characteristics of the stem cross-sections. (a–f) Toluidine blue staining of stem sections of a WT plant (a,d), PdOLP1-OE-transgenic plant (b,e) and PdOLP1-DR-transgenic plant (c,f). Scale bars = 100 µm. (g–i) Scanning electron microscopy of sections analyzed in WT (g), OE (h) and DR (i) plants. Scale bars = 5 µm. (j–l) Lignin autofluorescence was observed in WT (j), OE (k) and DR (l), Scale bars = 400 µm. (m–o) Phloroglucinol-HCl staining of WT (m), OE (n) and DR (o) plants. Scale bars = 400 µm. C, cortex cells; CZ, cambial zone; Pi, pith; P, secondary phloem; ve, vessel; X, secondary xylem; xf, xylem fiber.