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. 2020 Jun 10;21(11):4144. doi: 10.3390/ijms21114144

Figure 5.

Figure 5

Oxidative capacity and effects of IGF1 and IGFBP2 on lipid metabolism in hepatocytes. (A) Hepatocytes from C57Bl6 (C57), alb-SREBP-1c (alb), and aP2-SREBP-1c (aP2) mice were analyzed for palmitate oxidation in untreated cells or under inhibitors of mitochondrial activity (Oligomycin (Oligo), Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), Rotenone/Antimycin A (Rot/AA) and Etomoxir (Eto)) (B) palmitate oxidation, (C) palmitate uptake, and (D) de novo lipogenesis under basal conditions and in response to IGF1, IGFBP2, and IGF1 pre-bound to IGFBP2. The bar graphs represent mean (±95% CI; n = 4–9). Statistics: ANOVA with Sidak correction: †††, ††, and † indicate p < 0.001, p < 0.01 and p < 0.05 for the effects of IGF1; *** p < 0.001, ** p < 0.01 and * p < 0.05for the illustrated comparisons. Cpm: counts per minute.