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. 2020 May 27;21(11):3773. doi: 10.3390/ijms21113773

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Formation of chito-oligosaccharides (CHOS) from chitosan (0.5% w/v) by chitosanase-displaying L. plantarum cells harboring p^alrLp0373_CsnA_L at 37 °C (A,B) and 50 °C (C,D) using two different substrates: chitosan low molecular weight (A,C) and chitosan from crab shells medium molecular weight (B,D) as analysed by thin layer chromatography (TLC). Standards: commercial chito-oligosaccharides (TCI Deutschland GmBH, Eschborn, Germany) which contains chitobiose (C2), chitotriose (C3), chitotetraose (C4), chitopentose (C5), chitohexaose (C6) and D-glucosamine (GlcN).