Table 1.
Strains and plasmids used in this study.
| Strain or Plasmid | Relevant Characteristic (s) | Reference Source |
|---|---|---|
| Strains | ||
| L. plantarum | ||
| WCFS1 | wild type, host strain | [31] |
| TLG02 | Δalr, D-alanine auxotroph, food-grade expression host | [27] |
| E. coli | ||
| HST08 | cloning host | Clontech |
| MB2159 | D-alanine auxotroph, cloning host | [37] |
| Plasmids | ||
| pLp0373_ManB_S | Ermr; pSIP401 derivate encoding the Lp_0373 signal peptide translationally fused to manB-myc, followed by the short cell wall anchor (S) from Lp_1229 | (unpublished) |
| pLp0373_ManB_L | Ermr; pSIP401 derivate encoding the Lp_0373 signal peptide translationally fused to manB-myc, followed by the long cell wall anchor (L) from Lp_1229 | (unpublished) |
| pSIP409-CsnA-native | Ermr; spp- based expression vector pSIP409 for expression of csnA with native signal peptide | [11] |
| pEV | Ermr; pSIP401 derivative, empty vector, no signal sequence, no csnA (negative control) | [13] |
| pSIP603-GusA | Ermr; pSIP401 derivative, gusA controlled by PsppA, alr replaced erm | [27] |
| pLp0373_CsnA_S | Ermr; pLp0373_ManB_S derivative with csnA-myc instead of manB-myc | This study |
| pLp0373_CsnA_L | Ermr; pLp0373_ManB_L derivative with csnA-myc instead of manB-myc | This study |
| palrLp0373_CsnA_S | pSIP603 derivative with SPLp0373-csnA-myc-S instead of gusA | This study |
| palrLp0373_CsnA_L | pSIP603 derivative with SPLp0373-csnA-myc-L instead of gusA | This study |