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. 2020 Jun 8;21(11):4099. doi: 10.3390/ijms21114099

Figure 1.

Figure 1

The expression of Semaphorin3A (SEMA3A) is increased in podocytes from Doxorubicin (Dox)-induced renal injury mice. (A) Dual immunofluorescence staining of SEMA3A (red) and nephrin (green) in mouse glomeruli from the control, Dox, and Dox + SEMA3A-inhibitor (SEMA3A-I) groups at the time point of 2 weeks after Dox injection, showing the increase of SEMA3A expression and decrease of nephrin expression in Dox-treated podocytes. Representative images are shown. Original magnification, ×400. The nephrin and SEMA3A-positive area/glomeruli (%) are shown in the graphs. (B) Western blotting analysis of SEMA3A and β-actin in the control, Dox, and Dox + SEMA3A-I groups at the time point of 2 weeks after Dox injection, showing the increase of SEMA3A in the Dox group. Densitometric analysis was performed to quantify the Western blotting results. Data are shown from three independent experiments. ** p < 0.01, *** p < 0.001.