Inhibition of SEMA3A protected against Doxorubicin-induced podocyte injury and albuminuria. (A) Histological manifestations are determined by periodic acid-Schiff (PAS) staining to assess the glomerular injury in the control, Dox, and Dox + SEMA3A-I groups at the time point of 2 weeks after Dox injection. Glomerular structure and podocytes were severely damaged with tubular casts in the Dox group compared to the control and Dox + SEMA3A-I groups. Representative images are shown. Original magnification, ×200 (upper panel), ×400 (lower panel). The PAS-positive area/glomeruli (%), the ratio of glomeruli with sclerosis (%), and the number of tubular casts/200× fields are shown in the graphs. ** p < 0.01, *** p < 0.001. (B) Mouse urinary albumin/creatinine ratio in the control, Dox, and Dox + SEMA3A-I groups at the time point of 2 weeks after Dox injection, showing higher levels of urinary albumin in the Dox group compared to the control and Dox + SEMA3A-I groups. Analysis was conducted by the Wilcoxon test. * p < 0.05.