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. 2020 Jun 8;21(11):4099. doi: 10.3390/ijms21114099

Figure 5.

Figure 5

SEMA3A caused podocyte apoptosis while SEMA3A-inhibitor protected from podocyte apoptosis. (A) Representative immunofluorescence staining images of cleaved-caspase3 (C-Caspase3) (green) and DAPI (blue) in podocytes with or without Dox (0.5 μg/mL), SEMA3A (50 ng/mL), SEMA3A-I (0.5 μM), and Dox (0.5 μg/mL) + SEMA3A-I (0.5 μM). Original magnification, ×200. C-Caspase3-positive cells/nuclei (%) in 200× field are shown in the graphs. (B,C) RT-qPCR analysis of Bax and GAPDH mRNA with or without SEMA3A (50 ng/mL), SEMA3A-I (0.5 μM), Dox (0.5 μg/mL), Dox (0.5 μg/mL) + SEMA3A (50 ng/mL), and Dox (0.5 μg/mL) + SEMA3A-I (0.5 μM), showing that the mRNA level of Bax is increased with SEMA3A or Dox treatment, and is decreased with the SEMA3A-I treatment. * p < 0.05, ** p < 0.01, *** p < 0.001.