Table 1.
Summary of antibodies used for immunofluorescence and immunoblotting.
| Antigen | Manufacturer | Species | Dilution (IF) | Dilution (IB) |
|---|---|---|---|---|
| SEMA3A | Abcam | Rabbit | 1:100 | 1:1000 |
| Nephrin | Progen | Pig | 1:200 | - |
| C-Caspase3 | Cell Signaling | Rabbit | 1:300 | - |
| p-c-Jun | Cell Signaling | Rabbit | 1:200 | - |
| p-JNK | Cell Signaling | Rabbit | - | 1:1000 |
| JNK | Cell Signaling | Rabbit | - | 1:1000 |
| β-actin | Abcam | Rabbit | - | 1:2000 |
| GAPDH | Cell Signaling | Rabbit | - | 1:5000 |
IF, immunofluorescence; IB, immunoblotting; SEMA3A, Semaphorin3A; C-Caspase3, cleaved-Caspase3; p-c-Jun, phospho-c-Jun; p-JNK, phospho-c-Jun N-terminal kinase; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.