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. 2020 Jun 5;21(11):4043. doi: 10.3390/ijms21114043

Figure 6.

Figure 6

The effects of α-mangostin glycosides on the autophagy of Hep3B cells. (A) The effects of Man-3DG and Man-6DG on the expression of autophagy-related proteins in Hep3B cells. The cells were treated with Man-3DG and Man-6DG (10 and 20 μM) for 24 h, and the protein levels were detected through western blot analysis using specific antibodies. The levels of β-actin were used as an internal control. (B) The effects of α-mangostin, Man-3DG, and Man-6DG on Hep3B cell viability in combination with an autophagy inhibitor. Hep3B cells were treated with the three compounds (20 μM) in the presence or absence of 3-MA (1 mM) for 24 h. The cell viability was measured through MTT assay. Each value represents the mean ± SD from three independent experiments. ** p < 0.01 and *** p < 0.001 versus the control.