Table 1.
Primary sequence of the 40 variable bases (40Ns) of the M7 oligoaptamers
| apt lib | CCGGATCCGTTGATATAAAATTC(40Ns)TTGATTGTGGTGTTGGCTCCCAAGCTTCGG |
|---|---|
| M7-2 (70%) | TCTGGCTAGGATAGGCAGGAGTTTCATTACTTGTATGTTT |
| M7-1 | TCTGGCTAGGATAGGCAGGAGTTTCATTACTTATATGTTT |
| M7-3/M7-6 | TCCGGCTAGGATAGGCAGGAGTTTCATTACTTGTATGTTT |
| M7-13 | TCTGGCTAGGATAGGCAGGTGTTTCATTACTTGTATGTTT |
| M7-14 | TCTGGCTAGGATAGGCAGGAGCTTCATTATTTGTATGTTT |
| M7-22 | TCTGGCTAAGATAAACAAGGAGTTTCATTACTTGTATGTTT |
| M7-5 | TAGTCAGGTGAAGTCCGCGGCTAACAATTCAGTAGGTTA |
The ‘apt lib’ is the complete aptamer library of 1014–15 members, each containing 40 variable bases (40Ns) flanked by the forward and reverse primers on the left and right.
Nucleotides that deviate from the sequences of the identical clones are in bold.
M7-22 and M7-5 harbor 41 and 39 variable residues (Ns), respectively at their centers.
70% of the M7 clones share similar base sequence, as represented by the M7-2 clone.