Fig. 2.

SR-A and MARCO differentially bind to N. meningitidis surface proteins. Wells of duplicate 96-well plates were coated with 10 µg/ml of various purified His- or GST-tagged N. meningitidis surface proteins. a SR-A binding was detected using Mt lysates from WT and SR-A–/– BMMϕ along with the SR-A-specific monoclonal antibody 2F8. PBS and AcLDL were used as a negative and a positive control for binding to SR-A, respectively (inset). b Binding to MARCO was detected using recombinant soluble MARCO and the anti-MARCO monoclonal antibody ED31. Recombinant soluble EMR1 acted as a control for non-specific binding. ChSO₄ and DxSO₄ were used as a negative and a positive control for binding to MARCO, respectively (inset).