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. 2020 May 26;21(11):3750. doi: 10.3390/ijms21113750

Figure 4.

Figure 4

Increased responses to MSU crystals after in vivo transmigration or in vitro priming. (A) Tissue neutrophils derived from skin chambers (solid line) produced high amounts of intracellular ROS (icROS) in response to MSU crystals (300 µg/mL) as compared to blood neutrophils (dotted line) from the same donor. A representative kinetic curve out of three independent experiments is shown. Arrow indicates addition of stimuli. (B) In vitro priming of blood neutrophils (20 min, 37 °C) with IL-1β (100 ng/mL) or TNFα (10 ng/mL) mimicked in vivo priming; increased icROS responses were seen in the primed neutrophils as compared to resting control neutrophils when treated with MSU crystals (300 μg/mL). Peak values +/− SD are shown and a paired t-test was used for statistical comparisons. * p ≤ 0.05 and ** p ≤ 0.01. ns = not significant.