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. 2020 May 26;21(11):3750. doi: 10.3390/ijms21113750

Figure 5.

Figure 5

MSU crystals induce neutrophil extracellular trap (NET) formation. (A) Neutrophils stimulated with MSU crystals formed NETs as confirmed by DNA- (blue) and MPO- (green) labelled micrographs of neutrophils 3 h after stimulation with MSU crystals (300 µg/mL). Scale bar = 10 µm. (B) NET formation as measured with Sytox Green fluorescence over 3 h. To the left, a representative kinetic curve of neutrophils stimulated with buffer (black squares, broken line), MSU crystals (300 µg/mL, triangles, dotted line), or PMA (50 nM, black dots, solid line). MSU crystals (300 µg/mL) triggered a statistically significant DNA release (p = 0.0005 compared to buffer treated cells after 180 min, n = 12). To the right, NET formation evaluated after 3 h with different concentrations of MSU crystals, mean fluorescence +/− SEM are shown (n = 3). Statistical significance was calculated by the use of the Wilcoxon matched-pairs signed rank test.