Figure 1.
SerT knockdown induces a rough phenotype in the adult compound eye. The eye imaginal discs of wild-type third instar larvae were stained with 4′,6-diamidino-2-phenylindole (DAPI) to visualize the nucleus (A). The posterior region in the box stained with rabbit anti-Drosophila SerT antibody followed by anti-rabbit IgG Alexa FluorTM 594 antibody was used for detecting SerT (B), and the merged image is shown (B′). The images are representative of 10 eye imaginal discs. Scanning electron micrographs of the compound eyes of flies carrying GMR-Gal4/yw; +; + (C), GMR-Gal4/Y; UAS-SerT-IR1/+; +, (D), GMR-Gal4/Y; and UAS-SerT-IR2/+; + (E). Larger images of the boxed regions are also shown (C′–E′). Phenotypes were observed independently in at least three individuals of each fly lines, and no significant change was found in three individuals of the same line. Relative mRNA expression of SerT gene in eye discs of flies contains GMR > yw, GMR > SerTIR-1, and GMR > SerTIR-2, n = 5 (F). Scale bars indicate 100 μm (A,B,B′,C–E) and 30 μm (C′–E′). The triangles indicate MF. a, anterior; p, posterior; **** p < 0.0001