Table 2.
Summary of the impact of Notch signaling on Pref-1 expression and adipogenic differentiation.
| Cell Type | Experimental Approach | Impact on | References | ||
|---|---|---|---|---|---|
| Notch signaling | Adipogenic Differentiation | Pref-1 Expression | |||
| 3T3-L1 | Exposure to soluble Jagged1 | Up-regulated | Decreased | Not determined | [152] |
| hes1 over-expression | Up-regulated | Decreased | Not determined | ||
| hes1 knock-down | Down-regulated | Decreased | Up-regulated | ||
| 3T3-L1 | Notch4 over-expression | Up-regulated | Increased | Down-regulated | [149] |
| 3T3-L1 | Adipogenic differentiation. No specific treatment. | Decreases over time | Increased (compared to C3H10T1/2) | Not determined | [109] |
| dlk1 knock-down | Up-regulated | Not assessed | Down-regulated | ||
| C3H10T1/2 | Adipogenic differentiation. No specific treatment. | Remained unchanged | Decreased (compared to 3T3-L1 cells) | Up-regulated | |
| dlk1 over-expression | Down-regulated | Increased | Up-regulated | ||
| dlk1 knock-down | Up-regulated | Increased | Down-regulated | ||
| Notch1 knock-down | Down-regulated | Decreased | Down-regulated | ||
| dlk1 over-expression in Notch1 knock-down cells | Down-regulated | Decreased | Up-regulated | ||
| 3T3-L1 | Transfection with miRNA (miR-139–5p mimic) | Initially increased. Gradually decreased. | Decreased | Up-regulated | [154] |
| Co-transfection with pcDNA3.1_NICD (over-expressing Notch) | Up-regulated | Increased | Not determined | ||
| Mouse BM-derived MSCs | Inhibition of γ-secretase | Decreased | Increased | Decreased | [162] |
| Human BM-derived MSCs | Adipogenic differentiation. No specific treatment. | Decreased | Increased | Decreased | [158] |
| Inhibition of γ-secretase | Decreased | Increased | Not measured | ||
siRNA, small interfering RNA; miRNA, micro-RNA; BM, bone marrow