Figure 8.

Transcript levels of tumor necrosis factor (tnf-α) (A) interleukin 1β (il-1β) (B), and interleukin 10 (il-10) (C) in mature adipocytes incubated for 6 days with 100 µM oleic acid (OA) or 100 µM docosahexaenoic acid (DHA) and thereafter exposed to lipopolysaccharide (LPS) for 20 h (OA+LPS and DHA+LPS, respectively). Samples (n = 8 for OA and DHA groups and n = 6 for OA+LPS and DHA+LPS) were analyzed with real-time qPCR. Data are presented as fold change ± SEM using ef1α as a reference gene and the OA group was set to one (delta-delta method). Different letters indicate significant differences between treatments (p < 0.05, ANOVA followed by Tukey’s post hoc test).