Table 2.
Atlantic salmon primer sequences used for real-time PCR.
| Gene | Accession No. | Direction | Primer Sequence 5′→3′ |
|---|---|---|---|
| ef1α | AF321836 | Forward | CACCACCGGCCATCTGATCTACAA |
| Reverse | TCAGCAGCCTCCTTCTGAACTTC | ||
| etif3 | DW542195 | Forward | CAGGATGTTGTTGCTGGATGGG |
| Reverse | ACCCAACTGGGCAGGTCAAGA | ||
| β-actin | AF012125 | Forward | ACATCAAGGAGAAGCTGTGC |
| Reverse | GACAACGGAACCTCTCGTTA | ||
| fatp1 | CA373015 | Forward | TGGGAGCTTGTGGGTTCAA |
| Reverse | ACTTTCATGAGGCGGATTGG | ||
| mtp | CA042356.1 | Forward | CAAAGACCAGCGTCAACAACAA |
| Reverse | CGCCTCTGTCTCAAAGCTCACT | ||
| sod1 | BT057716 | Forward | TTCTGTTGTACGCTGTCCCAAAAGC |
| Reverse | GCAGCTTGGTACGCAAAGTGAACA | ||
| sod2 | BT060259 | Forward | TTCCGAGTGTGCCCAGTCTTGT |
| Reverse | ACAGGGAGGTGAAAGTGCATGGT | ||
| sod3 | BT046917 | Forward | TCATCGACAGCAGAGAAGAAGGGGA |
| Reverse | CTGCGATGAAAGGTGGTGAGCG | ||
| gpx1 | CA345853 | Forward | CCTTCCAGTACCTGGAGTTGAATGC |
| Reverse | CTCATGATTGTCTCCTGGCTCCTGT | ||
| tnf α | NM_001123589 | Forward | AGGTTGGCTATGGAGGCTGT |
| Reverse | TCTGCTTCAATGTATGGTGGG | ||
| il1 β | CA377361 | Forward | GTATCCCATCACCCCATCAC |
| Reverse | TTGAGCAGGTCCTTGTCCTT | ||
| il10 | EF165028 | Forward | ATGAGGCTAATGACGAGCTGGAGA |
| Reverse | GGTGTAGAATGCCTTCGTCCAACA | ||
| mfn1 | BT072406 | Forward | AGTGTGTCCAGTCTTCCGCACA |
| Reverse | ACAGGCTACAGCACCCAACCTT | ||
| fis1 | BT072691 | Forward | CCCCAGGGGGCATCCTGTCTTA |
| Reverse | TTGCAGCTGGCCGATCTAGCG | ||
| p53bp2 | XM014195885.1 | Forward | TTTTCCAGGCATCACCAATGAC |
| Reverse | CCAGATCAGCCATGATAGCGT |
Elongation factor 1A (ef1α), eukaryotic translation initiation factor 3 (etif3), fatty acid transport protein 1 (fatp1), microsomal triglyceride transfer protein (mtp), superoxide dismutase 1 (cytosolic) (sod1), superoxide dismutase 2 (mitochondrial) (sod2), superoxide dismutase 3 (extracellular) (sod3), glutathione peroxidase 1 (gpx1), tumor necrosis factor (tnf-α), interleukin 1β (il-1β), interleukin 10 (il-10), mitofusin 1 (mfn-1), mitochondrial fission 1 protein (fis-1), tumor protein P53 binding protein 2 (p53bp2).