Figure 2.
Effect of SYR on UVA-induced mRNA and protein expressions of MMPs, type I procollagen, and pro-inflammatory mediators in HaCaT keratinocytes. Cells were exposed to UVA radiation (10 J/cm2) and treated with given concentrations of SYR for 24 h. (A) mRNA expressions of MMP-1 and type I procollagen were analyzed with RT-PCR. (B) Protein levels of MMP-1, -9 and type I procollagen (C) and pro-inflammatory mediators (COX-2, TNF-α, iNOS, IL-1β and IL-6) were determined by Western blotting. β-Actin was used as an internal control for RT-PCR and Western blotting. Retinoic acid was used as a positive control.