Figure 3.

Effect of SYR on the total and phosphorylated (p-) protein levels of MAPK/AP-1 signaling effectors in UVA-irradiated HaCaT keratinocytes. Total cell lysates and nuclear extracts of the cells were used to determine the protein levels of phosphorylated (p-) and inactive forms of (A,B) MAPKs (p38, ERK and JNK), (C) c-Fos and c-Jun proteins by Western blotting. Cells were exposed to UVA radiation (10 J/cm²) and treated with or without given concentrations of SYR for 24 h (A,C), 6 h and 12 (B). β-Actin was used as an internal control for Western blotting. Lamin B1 was used as an internal control for nuclear fraction Western blotting.