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SDS-PAGE (non-reducing conditions, Coomassie staining) of flow-through (FT) and elution (EL) fractions obtained by purifying anti-ErbB2 affibody from a clarified E. coli cell lysate using a column packed with IGKQRI–GSG–Toyopearl resin. Labels: MW, molecular weight marker; L, load; UB, unbound; EL, elution at pH 3.8; Aff, affibody standard.
