Table 3.

Recovery and purity of anti-HSA and anti-IgG affibodies purified from clarified E. coli cell lysate using the adsorbent IGKQRI–GSG–Toyopearl, IHQRGQ–GSG–Toyopearl, KSAYHS–GSG–Toyopearl, and DIRIIR–GSG–Toyopearl resins. The values of yield were calculated as the ratio of the amount of fluorescently labeled affibody present in the eluted fractions (measured via fluorescence spectroscopy) vs. the loaded amount, whereas the values of purity were calculated by densitometric analysis of the elution (EL) lanes in the gel reported in Figure 1 using Image Lab software (Bio Rad).

Sequence	Anti-HSA Affibody		Anti-IgG Affibody
	Recovery	Purity	Recovery	Purity
IGKQRI	95.2% ± 4.6%	94.1%	97.2% ± 1.5%	95.8%
IHQRGQ	96.1% ± 2.4%	92.6%	95.3% ± 4.5%	93.1%
KSAYHS	89.5% ± 0.7%	96.3%	88.6% ± 3.4%	96.4%
DIRIIR	74.9% ± 3.6%	96.2%	78.3% ± 2.5%	96.5%