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Factor of amino-acid enrichment (fE) produced by the library screening, calculated as the ratio between the frequency of a group of amino acids in the identified sequences and the probability of finding it in a random peptide in the library.
| Amino Acid | Probability | Frequency | fE |
|---|---|---|---|
| Aliphatic (Ala, Ile, Gly) | 30% | 81% | 2.7 |
| Aromatic (Tyr) | 10% | 6% | −0.4 |
| Neutral hydrophilic | 20% | 69% | 3.5 |
| (Gln, Ser) + Charged | 30% | 100% | 3.3 |
| (His, Lys, Arg) − Charged (Asp) | 10% | 25% | 2.5 |
