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. 2020 Jun 11;21(11):4163. doi: 10.3390/ijms21114163

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transgenic rice over-expressing OsPYL/RCAR7 showed drought tolerance in vegetative stage. (A) Representative images of plants grown in the drought tolerance assay. All plants over-expressing OsPYL/RCAR7 (C14s) had the higher survival rate than wild type (WT) (DJs). Upper panel represents plants before drought stress treatment. Lower panel represents plants after drought stress treatment. (B) Graphical representation of survival rate after drought stress. Survival rate was calculated by counting the percentage of surviving plants in each pot. (C) Graphical representation of quantified fresh weight after drought stress. The graphs show average values with standard error (n = 6). The assay was conducted in triplicate and showed similar results. Plants were subjected to drought treatment for 7–10 days before recommencing watering. ** means p-value is less than 0.01,*** means p-value is less than 0.001 and **** means p-value is less than 0.0001.