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. 2020 Jun 11;21(11):4163. doi: 10.3390/ijms21114163

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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OsPYL/RCAR7 has stringent ABA dependency to interact with OsPP2CAs. (A) Yeast two hybrid analysis using OsPYL/RCAR7 and OsPYL/RCAR5 as baits and OsPP2CAs as preys. Synthetic minimal medium lacking leucine, tryptophan, and histidine (SD-LTH) supplemented with 0 to 5 μM concentration of ABA were used. Photographs were taken 5 days after inoculation of transformed yeasts. (B) OsPYL/RCAR7-GFP (green fluorescent protein) was immunoprecipiated and co-immunoprecipiated OsPP2C51 was detected by human influenza hemagglutinin (HA) antibody. (C) Inhibition assay of phosphatase activity for OsPPC50 was performed by three ABA receptors (OsPYL/RCAR3, 7, and 10).