OsPYL/RCAR7 showed the lowest activity among monomeric ABA receptors. (A) Comparative analysis of ABA signaling effects of monomeric ABA receptors under absence or presence of ABA. Luciferase (LUC) assay was done using rice protoplasts, reporter, and ABA receptors as signaling effectors. (B,C) Comparative analysis of OsPYL/RCAR7 and OsPYL/RCAR5 activity to suppress individual phosphatase PP2C08 and OsPP2C30. Luciferase assay was done using rice protoplasts, reporter, OsPP2CAs, and ABA receptors. Luciferase activity was measured by comparing with co-transformed marker (Ubi::rLUC), fLUC/rLUC. Values represent averages and error bars are SD (n = 3). These experiments were repeated three times. Results showed similar patterns and representative results were presented. – means that p-value is more than 0.05, * means that p-value is less than 0.05, ** means that p-value is less than 0.01 and **** means that p-value is less than 0.0001.