Table A2.
List of groups included in each experimental analysis.
| Experiment | Included Groups | |
|---|---|---|
| Dry eye assessment | Corneal staining | 8W, 1Y, and 2Y |
| Tear secretion | 8W, 1Y, and 2Y | |
| Conjunctival goblet cell count | 8W, 1Y, and 2Y | |
| Gross examination | MG area analyzed by meibography | 8W, 1Y, and 2Y |
| LG weight | 8W, 1Y, and 2Y | |
| MG analysis | Histology | |
| H&E staining | 8W, 1Y, and 2Y | |
| LipidTOX/PPARγ IHC (coronal section) * | 8W and 2Y | |
| Ki-67 and CD45 IHC * | 8W and 1Y | |
| 8-OHdG IHC * | 8W and 1Y | |
| Flow cytometry | ||
| 8-OHdGhiPIlo | 8W, 1Y, and 2Y | |
| BrdU+/7-AAD | 8W, 1Y, and 2Y | |
| LG analysis | Histology | |
| H&E staining and CD45 IHC | 8W, 1Y, and 2Y | |
| Ki-67 IHC | 8W, 1Y, and 2Y | |
| 8-OHdG IHC * | 8W and 1Y | |
| Flow cytometry | ||
| CD4+ IFNγ+ and CD4+ IL-17A+ | 8W, 1Y, and 2Y | |
| 8-OHdGhiPIlo | 8W, 1Y, and 2Y | |
| BrdU+/7-AAD | 8W, 1Y, and 2Y | |
| Secondary lymphoid organs | Flow cytometry of DLN (mice) | 8W, 1Y, and 2Y |
* Experiments performed in only two groups. Abbreviations: W, week-old; Y, year-old; MG, meibomian gland; LG, lacrimal gland; H&E, hematoxylin-eosin; IHC, immunohistochemical staining; DLN, drainage lymph node.