Figure 3.

(a) Treatment with the multi enzyme inhibitor RK-682 inhibited cell proliferation in MGH-U3, T24 cells and UROtsa. Cells were incubated in serum-free media for 24 h and treated with different concentrations of RK-682 for a further 48 h. The number of viable cells was measured by an MTS assay and expressed as a percentage of viable cells; (b) effect of RK-682 on MGH-U3 and T24 cells. RK-682 treatment resulted in a significant inhibition of MGH-U3 and T24 cell invasion (p < 0.05); (c) 48 h treatment with heparanase inhibitor RK-682 inhibited cell autophagy in MGH-U3 and T24 cells. The red horizontal line shows the range of LC3 intensity after KR-682 treatment.